Olympus/Picoquant FLCCS microscope
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Location | LCAM, Room A2.42 |
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| 2nd Contact person |  |
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| Techniques | (Semi-)Widefield imaging Confocal imaging Standard confocal techniques like co-localization & FRAP Fluorescence Fluctuation Spectroscopy (FFS) Confocal fluorescence lifetime microscopy (FLIM) Single-molecule analysis (SM) |
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| Booking | The microscope can be booked by trained users only. The schedule can be viewed in the webcalender. To book the microscope and for first time-users contact Mark Hink. | |
| Lasers | 405 nm diode laser (pulsing) 440 nm diode laser (pulsing) 478 nm, diode laser (pulsing) 561 nm dpss laser (CW) 560 nm diode laser (pulsing) 514 nm diode laser (CW) 633 nm HeNe laser (CW) other laser incoupling possibility locally or from laser room (>500 nm) For more info about laser safety, contact Ronald Breedijk (+7860) |
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| Objectives | 10x air UPLS Apo, N.A. 0.4 (wd 3.1 mm) 60x water immersed UPLS Apochromat, N.A. 1.2 (wd 0.28 mm) 60x silicon immersed UPLS Apochromat, N.A. 1.4 (wd 0.28 mm) |
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| Description: An Olympus Fluoview FV1000 confocal microscope, coupled to Picoquant’s pulsed laser diodes, MPD detectors and PicoHarp300 timing device for time-correlated single-photon counting. This machine is dedicated for confocal fluorescence lifetime imaging and fluorescence fluctuation spectroscopy. | Microscopes | Olympus IX81 inverted microscope FV1000 scan- and confocal detection head with three PMTs, Mercury and transmission lamp and transmission detector FITC/GFP (U-MNB2), CFP+YFP (U-M51017) and TRITC/RFP (U-MWG2) fluorescence cubes Prior motorized xyz stage Z-drift compensation system (ZDF) |
| Detection filters | Download filter-scheme here | |
| Accessoires | FV1000 fiber output to Picoquant detection unit Picoquant Fiber Coupling Unit II Picoquant Sepia II laser driver module Picoquant PH300 Custom-made PQ detection unit with 4 MPD Avalanche photodiodes Equiped with Nikon Okolab 37oC incubation box |
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| Software | Fluoview1000 files can be viewed with the Olympus viewer software or exported in other standard formats.
The Picoquant FCS/FLIM files can be processed using SymPhoTime 64 v1.5 software (dongle required), imported in SSTC’s FFS Dataprocessor or Matlab or converted by LCAM’s “ptu-converter” (check Links webpage) |
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| Manuals | LCAM Olympus Start-up manual LCAM user rules |