Leica Stellaris 8
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Location: | Sciencepark room A2.40 |
| Contact person: |  |
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| 2nd Contact person: |  |
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| Techniques: | Confocal imaging of max. 5 channels with standard, FLIM-optimised and/or red color-optimised HyDs (5x) (Semi-)Widefield imaging Standard confocal techniques like co-localization & FRAP FLIM & lifetime gated-imaging FRET N&B/RICS Fast overview imaging |
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| Booking: | The microscope can be booked by trained users only. The schedule can be viewed in the webcalender. First time-users should contact Mark Hink. | |
| Lasers: | 405 nm diode laser 440-790 pulsed White Light laser (25-80 MHz) For more info about laser safety, contact Ronald Breedijk (+7860) |
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| Objectives: | HC PL Fluotar 1.25x, NA0.04 (air) HC Plan Apo 10x, NA0.4 (air) HC Plan Apo 20x, NA0.75 (air) HC Plan Apo 63x, NA1.4 (oil) HC Plan Apo 100x, NA1.4 (oil) |
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| Filters: | Download laser and filter scheme here | |
| Accessoires: | Equiped with Okolab 37oC incubation box with option for CO2 Piezo element for precise z-stacking Adaptive focusing |
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| Software: | Leica Las-X, images can be imported into ImageJ using the LOCI plugin (automatically recognized in Fiji) | |
| Manuals: | LCAM Leica Stellaris8 Start-up manual LCAM user rules LCAM Manual connection to data-server Las-X helpfiles available via desktop link or via Mark |