Fluorescent proteins are widely used in biological research to make all kinds of cell types or structures visible. Think of, for example, stem cells or proteins related to cancer. A research team from the University of Amsterdam, led by …
Research highlight: UvA biologists develop new record bright red fluorescent protein Read more »
The vacuolar lumen of most plant cells is mildly acidic as compared to the surrounding cytoplasm, which is nearly neutral. In some plant tissues, however, vacuoles are extremely acidic. A few years ago, blue petunia flower mutants failing to hyper-acidify …
Pathogens use effector proteins to manipulate their hosts. During infection of tomato the fungus Fusarium oxysporum secretes the effectors Avr2 and Six5. Whereas Avr2 suffices to trigger I-2-mediated cell death in heterologous systems, both effectors are required for I-2-mediated disease …
Researchers succeeded for the first time in mapping protein-protein interactions in living developing plant roots. The findings of the international team of the department of Plant Developmental Biology at Wageningen University and Research, the section of Molecular Cytology at the …
Research highlight: Shedding light on protein interaction networks Read more »
The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and photostability, which are dependent on the characteristics of the fluorescent proteins that are employed. Yellow fluorescent protein (YFP) is often used as an acceptor but YFP is …
Research highlight: Spectrally diverse FRET acceptors for mTurquoise2 Read more »
In biological research, fluorescent proteins (FPs) are widely used in fluorescence microscopy. FPs are genetically encoded fluorophores, which are produced by cells after a noninvasive transfection. These proteins are utilized as markers and bio-sensors to study biological processes in living …
20.05.2016 European life science researchers now have the possibility to use state-of-the-art imaging technologies, which they do not find at their home institutions or among their collaboration partners. All they need to do is to apply to Euro-BioImaging (EuBI) via …
15 December 2015 The van Leeuwenhoek Centre for Advanced Microscopy (LCAM), a collaboration of the Netherlands Cancer Institute, the AMC-UvA and the UvA’s Faculty of Science, is according to Euro-BioImaging one of the leading microscopy centres in Europe. The centre …
The small GTPase RhoA is best known for its ability to remodel the actin cytoskeleton of cells. To study the mechanism underlying the spatiotemporal control of RhoA activity by GEFs, single cell imaging was performed with an improved FRET sensor reporting on the nucleotide loading …
Research highlight: Subcellular location of signaling proteins determines cell shape Read more »
Fluorescence lifetime imaging microscopy (FLIM) can be used to study protein-protein interactions by FRET or to perform lifetime-based multiplexing. Mostly live cell imaging is performed for FLIM imaging, but in combination with (antibody) stainings a fixation step is required. Nevertheless, …
Research highlight: The effect of sample fixation on fluorescence lifetime Read more »