Andor
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Location: | LCAM, room A2.42 |
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| 2nd Contact person: |  |
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| Techniques | (Semi-)Widefield imaging Confocal imaging (using spinning disk). Total Internal Reflection Microscopy (TIRF) Fluorescence Recovery After Photobleaching (FRAP) Photo-Activation Localization Microscopy (PALM) Multi-point confocal imaging (using Mosaic multimirror) |
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| Booking: | The Andor microscope can be booked via the webcalender and is only available for trained users. For bookings or first time-users contact Ronald Breedijk (+7860). | |
| Description: This Nikon TI microscope body can be equiped with multiple modular components like an Andor spinning disk and FRAPPA unit for confocal imaging, a laser setup with Optosplit and EMCCD cameras for super-resolution microscopy (PALM) or a multimirror device for widefield super-resolution imaging. The setup allows for laser incoupling under an angle, needed for total internal refelection microscopy (TIRF) or oblique angle illumination. | Lasers: | 405 nm 440 nm 488 nm 561 nm 594 nm (not available yet) For more info about laser safety, contact Ronald Breedijk (+7860) |
| Objectives: | 20x Plan Fluor, Extra long working distance N.A. 0.45, air 60x, N.A. 0.95, air 60x Apo TIRF, N.A. 1.49, oil 100x Apo TIRF, N.A. 1.49, oil |
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| Microscope: | Nikon Ti microscope + mercury and transmission lamp multiple fluorescence cubes (see detection filters) Yokogawa CSU X-1 motorized, triple dichroic spinning disk unit Andor FRAPPA unit 2x Andor iXon 897 CCD camera’s 3x Sutter excitation or emission filter wheels Andor Optosplit II dual color,-single channel unit |
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| Detection filters: | Download filter-scheme here | |
| Accessoires: | OKO-lab incubation chamber for temperature and CO2 control Prior motorized nanoscanZ stage + joystick Nikon perfect focusing system Nikon external phase contrast (for 60 & 100x TIRF) |
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| Software: | Andor files can be viewed with the Andor IQ 1.10.3 software (dongle required) or exported into other standard formats. For camera readout Andor Solis or Micromanager can be used. | |
| Manuals: | LCAM Andor Start-up manual LCAM user rules Nikon Ti manual (hardcopy only) Yokogawa manual (hardcopy only) Andor FRAPPA manual (hardcopy only) |