Zeiss LSM510
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Location: | LCAM, Room A2.40 |
| Contact person: |  |
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| 2nd Contact person: |  |
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| Techniques: | (Semi-)Widefield imaging Confocal imaging Standard confocal techniques like co-localization & FRAP Multi-Photon Excitation (MPE) UV excitation |
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| Booking: | The LSM510 microscope can be booked by trained users only. To book the microscope Ronald Breedijk (+7860) should be contacted. The measurement schedule will be published in the webcalender on Thursday, the week before. For first time-users contact Ronald Breedijk (+7860). | |
| Description: The Zeiss LSM510 is a four-channel confocal microscope also capable to perform UV- or MultiPhoton-excitation. | Lasers: | 355 (405) nm UV laser 445 nm diode laser 488 nm diode laser 514 nm diode laser 543 nm HeNe laser 561 nm diode laser 633 nm HeNe laser 700-960 nm Multi-photon laser For more info about laser safety, contact Ronald Breedijk (+7860) |
| Objectives: |
1. Plan Apochromat, 10x NA 0.30 (air) |
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| Microscope: | Axiovert 200M + mercury and transmission lamp CFP, FITC and TRITC fluorescence cubes motorized Marzhauser stage |
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| Detection filters: | Download filter-scheme | |
| Accessoires: | Small temperature box Multi-photon & UV excitation Fluorescence Correlation Spectroscopy (FCS) – bottom head |
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| Software: | LSM files can be viewed and processed with the LSM AIM software (dongle required), the free LSM ImageBrowser software or exported to other standard formats. The Loci plugin can be used to import the files into ImageJ or use the Fiji variant. |
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| Manual: | LCAM LSM510 manual LCAM LSM510 filter sheet LCAM user rules Zeiss LSM Meta manual (hardcopy only) |